出品公司: | BD Biosciences |
---|---|
载体名称: | pAcHLT A |
质粒类型: | 昆虫细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | -- |
启动子: | -- |
载体大小: | 8112 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | -- |
宿主细胞(系): | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
买家导航
pAcHLT A载体 pAcHLTA质粒基本信息
pAcHLT A质粒图谱 pAcHLTA载体图谱和pAcHLT A载体序列 pAcHLTA质粒序列多克隆位点信息
pAcHLT A质粒 pAcHLTA载体简介
pAcHLT A质粒序列 pAcHLTA载体序列
LOCUS pAcHLT A 8112 bp ds-DNA circular SYN 19-APR-2014 DEFINITION Baculovirus transfer vector for expressing proteins with an N-terminal 6xHis-PKA-thrombin cassette. For other reading frames, use pAcHLT B or pAcHLT C. ACCESSION . VERSION . KEYWORDS pAcHLT A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8112) AUTHORS BD Biosciences TITLE Direct Submission JOURNAL Exported Wednesday, February 5, 2020 from SnapGene Viewer 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8112 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" misc_recomb 1269..2096 /note="baculovirus recombination region (lef2/ORF603)" /note="contains ORF603 and part of lef2" CDS 1269..1393 /codon_start=3 /product="baculovirus late expression factor 2" /note="lef2" /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY" CDS complement(1431..2036) /codon_start=1 /product="baculovirus ORF603 protein" /note="ORF603" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR" promoter 2100..2191 /gene="polh from Autographa californica" /note="polyhedrin promoter" /note="promoter for the baculovirus polyhedrin gene" CDS 2230..2247 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" CDS 2254..2271 /codon_start=1 /product="recognition site for phosphorylation by protein kinase A" /note="PKA site" /translation="RRASVA" CDS 2281..2298 /codon_start=1 /product="thrombin recognition and cleavage site" /note="thrombin site" /translation="LVPRGS" misc_feature 2313..2384 /note="MCS" /note="multiple cloning site" misc_recomb 2404..3862 /note="baculovirus recombination region (ORF1629)" /note="contains part of ORF1629" CDS complement(2521..3862) /codon_start=2 /product="baculovirus capsid-associated protein" /note="ORF1629" /note="required for viral replication" /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA" rep_origin 5235..5690 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 5972..6076 /gene="bla" /note="AmpR promoter" CDS 6077..6937 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 7108..7696 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg 61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa 121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt 181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc 241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg 301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg 361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc 421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg 481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc 541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag 601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc 661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg 721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa 781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga 841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg 901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat 961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga 1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc 1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta 1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga 1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg 1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa 1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat 1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac 1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa 1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata 1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt 1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg 1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta 1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag 1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac 1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc 1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct 1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta 2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata 2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg 2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatagat 2221 ccgatgggac atcatcatca tcatcacgga aggagaaggg ccagtgttgc ggcgggaatt 2281 ttggtccctc gtggaagccc aggactcgat ggcatatgct cgatcgagga attcaggcct 2341 ccatgggagc tcgcggccgc ctgcagggta cccccgggag atctgtaccg actctgctga 2401 agaggaggaa attctccttg aagtttccct ggtgttcaaa gtaaaggagt ttgcaccaga 2461 cgcacctctg ttcactggtc cggcgtatta aaacacgata cattgttatt agtacattta 2521 ttaagcgcta gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa 2581 ttcattaaat ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc 2641 gtctttatat ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt 2701 agtttcaaac aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct 2761 caacgccaca aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg 2821 tgttttgttt tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct 2881 ttcatcactg tcgttagtgt acaattgact cgacgtaaac acgttaaata aagcttggac 2941 atatttaaca tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc 3001 gtcgttagaa gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt 3061 gtcggctaac acgtccgcga tcaaatttgt agttgagctt tttggaatta tttctgattg 3121 cgggcgtttt tgggcgggtt tcaatctaac tgtgcccgat tttaattcag acaacacgtt 3181 agaaagcgat ggtgcaggcg gtggtaacat ttcagacggc aaatctacta atggcggcgg 3241 tggtggagct gatgataaat ctaccatcgg tggaggcgca ggcggggctg gcggcggagg 3301 cggaggcgga ggtggtggcg gtgatgcaga cggcggttta ggctcaaatg tctctttagg 3361 caacacagtc ggcacctcaa ctattgtact ggtttcgggc gccgtttttg gtttgaccgg 3421 tctgagacga gtgcgatttt tttcgtttct aatagcttcc aacaattgtt gtctgtcgtc 3481 taaaggtgca gcgggttgag gttccgtcgg cattggtgga gcgggcggca attcagacat 3541 cgatggtggt ggtggtggtg gaggcgctgg aatgttaggc acgggagaag gtggtggcgg 3601 cggtgccgcc ggtataattt gttctggttt agtttgttcg cgcacgattg tgggcaccgg 3661 cgcaggcgcc gctggctgca caacggaagg tcgtctgctt cgaggcagcg cttggggtgg 3721 tggcaattca atattataat tggaatacaa atcgtaaaaa tctgctataa gcattgtaat 3781 ttcgctatcg tttaccgtgc cgatatttaa caaccgctca atgtaagcaa ttgtattgta 3841 aagagattgt ctcaagctcg gatcgatccc gcacgccgat aacaagcctt ttcattttta 3901 ctacagcatt gtagtggcga gacacttcgc tgtcgtcgac gtacatgtat gctttgttgt 3961 caaaaacgtc gttggcaagc tttaaaatat ttaaaagaac atctctgttc agcaccactg 4021 tgttgtcgta aatgttgttt ttgataattt gcgcttccgc agtatcgaca cgttcaaaaa 4081 attgatgcgc atcaattttg ttgttcctat tattgaataa ataagattgt acagattcat 4141 atctacgatt cgtcatggcc accacaaatg ctacgctgca aacgctggta caattttacg 4201 aaaactgcaa aaacgtcaaa actcggtata aaataatcaa cgggcgcttt ggcaaaatat 4261 ctattttatc gcacaagccc actagcaaat tgtatttgca gaaaacaatt tcggcgcaca 4321 attttaacgc tgacgaaata aaagttcacc agttaatgag cgaccaccca aattttataa 4381 aaatctattt taatcacggt tccatcaaca accaagtgat cgtgatggac tacattgact 4441 gtcccgattt atttgaaaca ctacaaatta aaggcgagct ttcgtaccaa cttgttagca 4501 atattattag acagctgtgt gaagcgctca acgatttgca caagcacaat ttcatacaca 4561 acgacataaa actcgaaaat gtcttatatt tcgaagcact tgatcgcgtg tatgtttgcg 4621 attacggatt gtgcaaacac gaaaactcac ttagcgtgca cgacggcacg ttggagtatt 4681 ttagtccgga aaaaattcga cacacaacta tgcacgtttc gtttgactgg tacgcggcgt 4741 gttaacatac aagttgctaa ccggcggccg acacccattt gaaaaaagcg aagacgaaat 4801 gttggacttg aatagcatga agcgtcgtca gcaatacaat gacattggcg ttttaaaaca 4861 cgttcgtaac gttaacgctc gtgactttgt gtactgccta acaagataca acatagattg 4921 tagactcaca aattacaaac aaattataaa acatgagttt ttgtcgtaaa aatgccactt 4981 gttttacgag tagaattagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa 5041 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 5101 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 5161 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc 5221 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 5281 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 5341 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 5401 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg 5461 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 5521 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta 5581 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 5641 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac 5701 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 5761 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 5821 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 5881 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 5941 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 6001 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 6061 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 6121 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 6181 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 6241 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 6301 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 6361 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 6421 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 6481 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 6541 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 6601 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 6661 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 6721 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 6781 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 6841 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 6901 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 6961 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 7021 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 7081 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 7141 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 7201 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 7261 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 7321 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 7381 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 7441 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 7501 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 7561 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 7621 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 7681 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 7741 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 7801 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 7861 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 7921 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 7981 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 8041 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 8101 tgattacgaa tt //