| 出品公司: | BD Biosciences |
|---|---|
| 载体名称: | pAcHLT A |
| 质粒类型: | 昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 8112 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pAcHLTA载体基本信息
pAcHLTA载体图谱质粒图谱和多克隆位点信息
pAcHLTA载体简介
pAcHLTA载体序列
LOCUS pAcHLT A 8112 bp ds-DNA circular SYN 19-APR-2014
DEFINITION Baculovirus transfer vector for expressing proteins with an
N-terminal 6xHis-PKA-thrombin cassette. For other reading frames,
use pAcHLT B or pAcHLT C.
ACCESSION .
VERSION .
KEYWORDS pAcHLT A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8112)
AUTHORS BD Biosciences
TITLE Direct Submission
JOURNAL Exported Wednesday, February 5, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8112
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
misc_recomb 1269..2096
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 1269..1393
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(1431..2036)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
promoter 2100..2191
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
CDS 2230..2247
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 2254..2271
/codon_start=1
/product="recognition site for phosphorylation by protein
kinase A"
/note="PKA site"
/translation="RRASVA"
CDS 2281..2298
/codon_start=1
/product="thrombin recognition and cleavage site"
/note="thrombin site"
/translation="LVPRGS"
misc_feature 2313..2384
/note="MCS"
/note="multiple cloning site"
misc_recomb 2404..3862
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(2521..3862)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin 5235..5690
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5972..6076
/gene="bla"
/note="AmpR promoter"
CDS 6077..6937
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7108..7696
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatagat
2221 ccgatgggac atcatcatca tcatcacgga aggagaaggg ccagtgttgc ggcgggaatt
2281 ttggtccctc gtggaagccc aggactcgat ggcatatgct cgatcgagga attcaggcct
2341 ccatgggagc tcgcggccgc ctgcagggta cccccgggag atctgtaccg actctgctga
2401 agaggaggaa attctccttg aagtttccct ggtgttcaaa gtaaaggagt ttgcaccaga
2461 cgcacctctg ttcactggtc cggcgtatta aaacacgata cattgttatt agtacattta
2521 ttaagcgcta gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa
2581 ttcattaaat ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc
2641 gtctttatat ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt
2701 agtttcaaac aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct
2761 caacgccaca aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg
2821 tgttttgttt tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct
2881 ttcatcactg tcgttagtgt acaattgact cgacgtaaac acgttaaata aagcttggac
2941 atatttaaca tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc
3001 gtcgttagaa gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt
3061 gtcggctaac acgtccgcga tcaaatttgt agttgagctt tttggaatta tttctgattg
3121 cgggcgtttt tgggcgggtt tcaatctaac tgtgcccgat tttaattcag acaacacgtt
3181 agaaagcgat ggtgcaggcg gtggtaacat ttcagacggc aaatctacta atggcggcgg
3241 tggtggagct gatgataaat ctaccatcgg tggaggcgca ggcggggctg gcggcggagg
3301 cggaggcgga ggtggtggcg gtgatgcaga cggcggttta ggctcaaatg tctctttagg
3361 caacacagtc ggcacctcaa ctattgtact ggtttcgggc gccgtttttg gtttgaccgg
3421 tctgagacga gtgcgatttt tttcgtttct aatagcttcc aacaattgtt gtctgtcgtc
3481 taaaggtgca gcgggttgag gttccgtcgg cattggtgga gcgggcggca attcagacat
3541 cgatggtggt ggtggtggtg gaggcgctgg aatgttaggc acgggagaag gtggtggcgg
3601 cggtgccgcc ggtataattt gttctggttt agtttgttcg cgcacgattg tgggcaccgg
3661 cgcaggcgcc gctggctgca caacggaagg tcgtctgctt cgaggcagcg cttggggtgg
3721 tggcaattca atattataat tggaatacaa atcgtaaaaa tctgctataa gcattgtaat
3781 ttcgctatcg tttaccgtgc cgatatttaa caaccgctca atgtaagcaa ttgtattgta
3841 aagagattgt ctcaagctcg gatcgatccc gcacgccgat aacaagcctt ttcattttta
3901 ctacagcatt gtagtggcga gacacttcgc tgtcgtcgac gtacatgtat gctttgttgt
3961 caaaaacgtc gttggcaagc tttaaaatat ttaaaagaac atctctgttc agcaccactg
4021 tgttgtcgta aatgttgttt ttgataattt gcgcttccgc agtatcgaca cgttcaaaaa
4081 attgatgcgc atcaattttg ttgttcctat tattgaataa ataagattgt acagattcat
4141 atctacgatt cgtcatggcc accacaaatg ctacgctgca aacgctggta caattttacg
4201 aaaactgcaa aaacgtcaaa actcggtata aaataatcaa cgggcgcttt ggcaaaatat
4261 ctattttatc gcacaagccc actagcaaat tgtatttgca gaaaacaatt tcggcgcaca
4321 attttaacgc tgacgaaata aaagttcacc agttaatgag cgaccaccca aattttataa
4381 aaatctattt taatcacggt tccatcaaca accaagtgat cgtgatggac tacattgact
4441 gtcccgattt atttgaaaca ctacaaatta aaggcgagct ttcgtaccaa cttgttagca
4501 atattattag acagctgtgt gaagcgctca acgatttgca caagcacaat ttcatacaca
4561 acgacataaa actcgaaaat gtcttatatt tcgaagcact tgatcgcgtg tatgtttgcg
4621 attacggatt gtgcaaacac gaaaactcac ttagcgtgca cgacggcacg ttggagtatt
4681 ttagtccgga aaaaattcga cacacaacta tgcacgtttc gtttgactgg tacgcggcgt
4741 gttaacatac aagttgctaa ccggcggccg acacccattt gaaaaaagcg aagacgaaat
4801 gttggacttg aatagcatga agcgtcgtca gcaatacaat gacattggcg ttttaaaaca
4861 cgttcgtaac gttaacgctc gtgactttgt gtactgccta acaagataca acatagattg
4921 tagactcaca aattacaaac aaattataaa acatgagttt ttgtcgtaaa aatgccactt
4981 gttttacgag tagaattagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
5041 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
5101 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
5161 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
5221 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
5281 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
5341 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
5401 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
5461 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
5521 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
5581 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
5641 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
5701 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
5761 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
5821 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
5881 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
5941 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
6001 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
6061 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
6121 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
6181 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
6241 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
6301 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
6361 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
6421 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
6481 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
6541 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
6601 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
6661 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
6721 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
6781 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
6841 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
6901 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
6961 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
7021 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
7081 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
7141 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
7201 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
7261 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
7321 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
7381 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
7441 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
7501 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
7561 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
7621 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
7681 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
7741 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
7801 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
7861 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
7921 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
7981 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
8041 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
8101 tgattacgaa tt
//
